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KMID : 1161320230380020054
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Journal of Animal Reproduciton and Biotechnology
2023 Volume.38 No. 2 p.54 ~ p.61
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Supplementation of retinoic acid alone in MSC culture medium induced germ cell-like cell differentiation
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Kuldeep Kumar
Kinsuk Das
Ajay Kumar
Purnima Singh
Madhusoodan A. P.
Triveni Dutt
Sadhan Bag
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Abstract
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Background: Germ cells undergo towards male or female pathways to produce spermatozoa or oocyte respectively which is essential for sexual reproduction. Mesenchymal stem cells (MSCs) have the potential of trans-differentiation to the multiple cell lineages.
Methods: Herein, rat MSCs were isolated from bone marrow and characterized by their morphological features, expression of MSC surface markers, and in-vitro differentiation capability.
Results: Thereafter, we induced these cells only by retinoic acid supplementation in MSC medium and, could able to show that bone marrow derived MSCs are capable to trans-differentiate into male germ cell-like cells in-vitro. We characterized these cells by morphological changes, the expressions of germ cell specific markers by immunophenotyping and molecular biology tools. Further, we quantified these differentiated cells.
Conclusions: This study suggests that only Retinoic acid in culture medium could induce bone marrow MSCs to differentiate germ cell-like cells in-vitro. This basic method of germ cell generation might be helpful in the prospective applications of this technology.
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KEYWORD
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differentiation, germ cell-like cells, mesenchyamal stem cells, retinoic acid
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